Nanoclew Synthesis Sequence Design Ligation Optimization Sizing and Characterization Duplex Formation Optimization + Verification Secondary Structure Folding Annealing Duplexes to Nanoclews Drug Loading Doxorubicin Loading and Release ZnPC Loading + ROS Generation Structure Binding Aptamer Binding Confirmation Endocytosis Endocytosis Verification Cell Viability Assessment Appendix: Cell Maintenance
