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Nanoclew Synthesis
Sequence Design
Ligation Optimization
Sizing and Characterization
Duplex Formation
Optimization + Verification
Secondary Structure Folding
Annealing Duplexes to Nanoclews
Drug Loading
Doxorubicin Loading and Release
ZnPC Loading + ROS Generation
Cell Experiments
Aptamer Binding Confirmation
Endocytosis Verification
Cell Viability Assessment
Cell Maintenance
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